Deficit irrigation strategies (PRD, SDI) and titanium nanoparticles improve water use efficiency and flower quality in greenhouse-grown cut roses.

This study explored the use of deficit irrigation techniques for water management in the hydroponic greenhouse cultivation of cut roses. A factorial experiment was conducted using three irrigation treatments: full irrigation (FI), partial root drying (PRD), and sustained deficit irrigation (SDI), and three doses of titanium dioxide nanoparticle foliar application (0, 15, and 30 ppm) as stress alleviation. Results showed that drought stress increased biochemical parameters such as the plants' proline and total phenol content. Compared to SDI treatment, the PRD treatments have an increase in flower number by 40%. The PRD strategy has positive effects on drought tolerance by increasing osmotic and elastic adjustment. Therefore, higher relative water content and longer root length in PRD treatments were observed. Thus, Biomass water use efficiency significantly increased in PRD treatments compared to others. In the PRD treatment, yield WUE increases by 26% and 61% compared to FI and SDI, respectively. The results showed TiO2-NPs positively affected mitigating and even improving some traits in drought stress conditions. These results suggest the superiority of the PRD strategy, which improves growth characteristics and water use efficiency, leading to increased sustainability, reduced environmental impact of greenhouse toxic wastewater, and total profitability of the greenhouse.

Phylogenomics revealed migration routes and adaptive radiation timing of Holarctic malaria mosquito species of the Maculipennis Group.

BACKGROUND: Phylogenetic analyses of closely related species of mosquitoes are important for better understanding the evolution of traits contributing to transmission of vector-borne diseases. Six out of 41 dominant malaria vectors of the genus Anopheles in the world belong to the Maculipennis Group, which is subdivided into two Nearctic subgroups (Freeborni and Quadrimaculatus) and one Palearctic (Maculipennis) subgroup. Although previous studies considered the Nearctic subgroups as ancestral, details about their relationship with the Palearctic subgroup, and their migration times and routes from North America to Eurasia remain controversial. The Palearctic species An. beklemishevi is currently included in the Nearctic Quadrimaculatus subgroup adding to the uncertainties in mosquito systematics. RESULTS: To reconstruct historic relationships in the Maculipennis Group, we conducted a phylogenomic analysis of 11 Palearctic and 2 Nearctic species based on sequences of 1271 orthologous genes. The analysis indicated that the Palearctic species An. beklemishevi clusters together with other Eurasian species and represents a basal lineage among them. Also, An. beklemishevi is related more closely to An. freeborni, which inhabits the Western United States, rather than to An. quadrimaculatus, a species from the Eastern United States. The time-calibrated tree suggests a migration of mosquitoes in the Maculipennis Group from North America to Eurasia about 20-25 million years ago through the Bering Land Bridge. A Hybridcheck analysis demonstrated highly significant signatures of introgression events between allopatric species An. labranchiae and An. beklemishevi. The analysis also identified ancestral introgression events between An. sacharovi and its Nearctic relative An. freeborni despite their current geographic isolation. The reconstructed phylogeny suggests that vector competence and the ability to enter complete diapause during winter evolved independently in different lineages of the Maculipennis Group. CONCLUSIONS: Our phylogenomic analyses reveal migration routes and adaptive radiation timing of Holarctic malaria vectors and strongly support the inclusion of An. beklemishevi into the Maculipennis Subgroup. Detailed knowledge of the evolutionary history of the Maculipennis Subgroup provides a framework for examining the genomic changes related to ecological adaptation and susceptibility to human pathogens. These genomic variations may inform researchers about similar changes in the future providing insights into the patterns of disease transmission in Eurasia.

Impact of dyadic practice on the clinical self-efficacy and empathy of nursing students.

BACKGROUND: Dyadic practice of learners creates supportive learning. So far, few studies have investigated the impact of this approach on students' empathy and self-efficacy. This study aimed to investigate the effect of dyadic practice on nursing students' clinical self-efficacy and empathy. METHODS: This study was based on a pretest-posttest randomized group from September to December 2018. All the junior nursing students (n = 44) were divided into intervention (n = 22) and control groups (n = 22) using stratified random sampling. The intervention group was trained for 6 days as student dyads, while the control group was under the supervision of an instructor and worked individually. The students' levels of empathy and self-efficacy were evaluated on the first day (pretest) and the last day (post-test) by The Self-Efficacy in Clinical Performance Scale and Mehrabian and Epstein empathy scale. The data were analyzed using the SPSS software by Fisher's exact test, Mann-Whitney test, independent t-test paired t-test, Wilcoxon signed-rank, and Analysis of covariance. RESULTS: Dyadic practice increased empathy in the intervention group compared to the control group (P < 0.001). The adjusted mean of total empathy in the intervention group was 21.1 degrees higher than the adjusted mean of total empathy in the control group. However, no significant differences were found between the two groups in clinical self-efficacy (P = 0.762). CONCLUSIONS: The employment of this approach seems helpful in creating an empathic atmosphere. However, further studies are required to prove the effectiveness of this method on self-efficacy.

Psychological Impact of COVID-19 on University Students in Bahrain.

Background COVID-19 is an ongoing event that has affected millions worldwide financially, socially, and psychologically; university students have also felt the devastating impact. Therefore, assessing how students have been affected psychologically is important, which is explored in this study. Methods This cross-sectional study took place in two institutions in Bahrain between October and December 2021. A survey assembled from pre-validated questionnaires was sent through Google Forms after receiving ethics approval. Data were analyzed using network plot analysis and linear regression analysis. Results Out of 292 responses, the most common demographic observed were vaccinated Bahraini females living with families at a mean age of 21.21 (SD±3.447). The mean scores for the Generalized Anxiety Disorder Scale (GAD-7), Centered for Epidemiological Studies-Depression scale (CES-D), and Stigma Scale were 10.55 (SD ± 6.144), 18.75 (SD ± 9.147), and 25.40 (SD ± 3.428), respectively. The significant associations on ANOVA observed were stigma score and living status (p-value = 0.002), stigma, and alcohol consumption (p-value = <0.001). Statistically significant predictors of the outcomes in this study included gender for depressive symptoms (p-value = 0.013) and alcohol consumption for stigma (p-value = 0.017). Discussion The stigmatization of the pandemic may additionally influence sub-threshold depressive and anxiety-related symptoms in university students. Concluding from the results of this study, the psychological impact of the pandemic is significant among university students and needs to be addressed in institutions in Bahrain.

A Review on the Incidence and Related Risk Factors of Retinopathy of Prematurity Across Various Countries.

Retinopathy of prematurity (ROP) is an ophthalmologic condition that is one of the leading causes of preventable childhood blindness. Due to the premature nature of blood vessels in preterm infants, retinal vessels are prone to damage. The incidence of ROP ranges with great variation across countries, and this study aims to identify the incidence and its related risk factors. A compilation of studies investigating the incidence of ROP was acquired through the PubMed and Google Scholar databases with a full free text in English filter set. All members of the study were involved in designating studies based on continent and arranging them into a table format. Following this, reasons for the variation in the incidence of ROP were investigated by individually assessing each study. The variation of an increased incidence of ROP seen in low-income countries (LICs) was most likely due to the availability of resources. As a preterm birth can give rise to further complications such as the development of sepsis, it is important to manage preterm birth with the utmost caution. Evidence has suggested that the two key variables in reducing the morbidity and mortality of ROP are the implementation of a screening and treatment protocol with controlled use of oxygen and the availability of resources in hospitals to adequately identify and manage ROP as early as possible. Through a comprehensive overview of the incidence of ROP, it is vital to review screening and treatment protocols in each hospital for ROP, which would aid in preventing its occurrence and initiating early treatment.

Evolutionary superscaffolding and chromosome anchoring to improve Anopheles genome assemblies.

BACKGROUND: New sequencing technologies have lowered financial barriers to whole genome sequencing, but resulting assemblies are often fragmented and far from 'finished'. Updating multi-scaffold drafts to chromosome-level status can be achieved through experimental mapping or re-sequencing efforts. Avoiding the costs associated with such approaches, comparative genomic analysis of gene order conservation (synteny) to predict scaffold neighbours (adjacencies) offers a potentially useful complementary method for improving draft assemblies. RESULTS: We evaluated and employed 3 gene synteny-based methods applied to 21 Anopheles mosquito assemblies to produce consensus sets of scaffold adjacencies. For subsets of the assemblies, we integrated these with additional supporting data to confirm and complement the synteny-based adjacencies: 6 with physical mapping data that anchor scaffolds to chromosome locations, 13 with paired-end RNA sequencing (RNAseq) data, and 3 with new assemblies based on re-scaffolding or long-read data. Our combined analyses produced 20 new superscaffolded assemblies with improved contiguities: 7 for which assignments of non-anchored scaffolds to chromosome arms span more than 75% of the assemblies, and a further 7 with chromosome anchoring including an 88% anchored Anopheles arabiensis assembly and, respectively, 73% and 84% anchored assemblies with comprehensively updated cytogenetic photomaps for Anopheles funestus and Anopheles stephensi. CONCLUSIONS: Experimental data from probe mapping, RNAseq, or long-read technologies, where available, all contribute to successful upgrading of draft assemblies. Our evaluations show that gene synteny-based computational methods represent a valuable alternative or complementary approach. Our improved Anopheles reference assemblies highlight the utility of applying comparative genomics approaches to improve community genomic resources.

Cognitive Behavioral Therapy for Occupational Stress among the Intensive Care Unit Nurses.

BACKGROUND AND AIMS: Nurses working in the intensive care units (ICU) are faced with numerous stressors that can pose a serious threat to their self-efficacy and affect the quality of care. The aim of this study was to evaluate the effectiveness of Cognitive Behavioral Therapy (CBT) on the ICU nurses' occupational stress. METHODS: This interventional study was conducted in a hospital in southeast of Iran. The participants included 120 nurses, who were randomly assigned into the control (n = 60) and intervention (n = 60) groups. In the pre-test, the occupational stress was assessed using Osipow questionnaire. Later, the intervention group attended the CBT course conducted in six 90 minute sessions. One month after the intervention, the post-test data were collected from both groups. RESULTS: The means of occupational stress and its dimensions were not significant before the intervention between the intervention and control groups (P = 0.47). The means of occupational stress and its dimensions were moderately high at pretest for all nurses. In the post-test, the stress level and all its dimensions reduced from moderate-high to moderate-low (P < 0.001), except for the physical environment dimension that remained at the moderate-high level (P = 0.32). CONCLUSION: The findings showed that CBT was effective on the nursing stress. Therefore, CBT training is suggested in in-service training programs for nurses.

Time to task failure of the contralateral untrained limb after high load-low repetition eccentric and low load-high repetition resistance training

Abstract Aims: Cross-training is the process whereby training of one limb gives rise to enhancements in the performance of the opposite, untrained limb and may be dependent on type of muscle contractions performed. The aim of this study was to investigate whether unilateral resistance training using eccentric contraction is more effective than concentric resistance training to improve time to task failure in the contralateral untrained limb. Methods:Subjects completed 12 weeks of resistance training consisting of 36 sessions, using unilateral leg exercise. Sustained isometric knee extension performed at 50% of maxmal force until task failure for the contralateral untrained leg. Surface electromyography (EMG) signals were simultaneously recorded from contralateral untrained quadriceps (vastusmedialis, rectus femoris, and vastuslateralis). Results: Time to task failure of the contralateraluntrained leg and associated EMG activitiessignificantly increased after 12 weeks ofunilateral resistance training(p<0.05). However, percent increase in time to task failure and EMG amplitude after eccentric resistance training was significantly higher than concentric resistance training (p<0.05). Conclusion: This study concluded that unilateral eccentric resistancetraining is superior to concentric resistance training to increase time to task failure in the contralateral untrained limb.(AU)

Complications in neonates of mothers with gestational diabetes mellitus receiving insulin therapy versus dietary regimen.

BACKGROUND: Gestational diabetes mellitus (GDM) is a common obstetrical complication with both maternal and fetal side effects. OBJECTIVE: This study was performed to determine the complications in neonates of mothers with GDM receiving insulin vs. dietary regimen. MATERIALS AND METHODS: In this prospective cohort study, 140 neonates of mothers with GDM attending Javaheri Hospital of Azad University in Tehran in 2013 and 2014 were enrolled and the complications in those receiving insulin versus. dietary regimen were compared. RESULTS: The results demonstrated that 95.7% of those who received a dietary regimen and 85.7% among those who received insulin had a good outcome showing statistically significant differences (p=0.042). The mortality rate was not differed among the patients in two groups (p>0.05). CONCLUSION: According to the results of this study, it may be concluded that the frequency of complications in neonates of cases with GDM is getting less by receiving dietary regimen.

Mosquito genomics. Highly evolvable malaria vectors: the genomes of 16 Anopheles mosquitoes.

Variation in vectorial capacity for human malaria among Anopheles mosquito species is determined by many factors, including behavior, immunity, and life history. To investigate the genomic basis of vectorial capacity and explore new avenues for vector control, we sequenced the genomes of 16 anopheline mosquito species from diverse locations spanning ~100 million years of evolution. Comparative analyses show faster rates of gene gain and loss, elevated gene shuffling on the X chromosome, and more intron losses, relative to Drosophila. Some determinants of vectorial capacity, such as chemosensory genes, do not show elevated turnover but instead diversify through protein-sequence changes. This dynamism of anopheline genes and genomes may contribute to their flexible capacity to take advantage of new ecological niches, including adapting to humans as primary hosts.

Effect of education on quality of life of family caregivers of children with leukemia referred to the Oncology Clinic at Kerman's Afzali-Poor Hospital (Iran), 2012.

OBJECTIVE: To investigate the impact of education on the quality of life of parent of children with leukemia referred to the oncology clinic at Kerman's Afzali-Poor hospital (Iran). METHODOLOGY: This was an interventional study with 80 parents of children with leukemia; all of the caregivers were mothers and were included in the research. They were chosen using the convenience sampling method and categorized randomly into two groups (intervention and control, each with 40 participants). Research tools included demographic information and Quality of Life (QoL) scale (a specific form for first-degree family caregivers of patients with leukemia). Questionnaires were applied at the beginning of the research and after three months; this second evaluation coincided in the study group with the end of the intervention. The intervention lasted four sessions of 45- to 65-minute classes, with lectures and question and answer sessions, educational booklets, and posters in groups with 4 - 6 members. RESULTS: In the first evaluation, mean QoL scores in the study and control groups were 224.9 and 225.7, which, after three months changed to 338.2 and 226.7, respectively. T-test verified these increases (p<0.05) in quality of life in the intervention group. CONCLUSION: According to the effectiveness of the education on the parent's quality of life, it is recommended to implement consulting and educational programs for parents, especially parents of children with leukemia, to promote care, reduce anxiety, and consequently, enhance quality of life.

Multigene phylogenetics reveals temporal diversification of major African malaria vectors.

The major vectors of malaria in sub-Saharan Africa belong to subgenus Cellia. Yet, phylogenetic relationships and temporal diversification among African mosquito species have not been unambiguously determined. Knowledge about vector evolutionary history is crucial for correct interpretation of genetic changes identified through comparative genomics analyses. In this study, we estimated a molecular phylogeny using 49 gene sequences for the African malaria vectors An. gambiae, An. funestus, An. nili, the Asian malaria mosquito An. stephensi, and the outgroup species Culex quinquefasciatus and Aedes aegypti. To infer the phylogeny, we identified orthologous sequences uniformly distributed approximately every 5 Mb in the five chromosomal arms. The sequences were aligned and the phylogenetic trees were inferred using maximum likelihood and neighbor-joining methods. Bayesian molecular dating using a relaxed log normal model was used to infer divergence times. Trees from individual genes agreed with each other, placing An. nili as a basal clade that diversified from the studied malaria mosquito species 47.6 million years ago (mya). Other African malaria vectors originated more recently, and independently acquired traits related to vectorial capacity. The lineage leading to An. gambiae diverged 30.4 mya, while the African vector An. funestus and the Asian vector An. stephensi were the most closely related sister taxa that split 20.8 mya. These results were supported by consistently high bootstrap values in concatenated phylogenetic trees generated individually for each chromosomal arm. Genome-wide multigene phylogenetic analysis is a useful approach for discerning historic relationships among malaria vectors, providing a framework for the correct interpretation of genomic changes across species, and comprehending the evolutionary origins of this ubiquitous and deadly insect-borne disease.

Effect of education on quality of life of family caregivers of children with leukemia referred to the Oncology Clinic at Kerman's Afzali-Poor Hospital (Iran), 2012 / Efecto de la educación en la calidad de vida de los cuidadores familiares de menores con leucemia referidos a la Clínica Oncológica en el hospital Afzali-Poor en Kerman (Iran), 2012 / Efeito da educação na qualidade de vida dos cuidadores familiares de menores com leucemia referidos à Clínica Oncológica no hospital Afzali-Poor em Kerman (Iran), 2012

Objective. To investigate the impact of education on the quality of life of parent of children with leukemia referred to the oncology clinic at Kerman's Afzali-Poor hospital (Iran). Methodology. This was an interventional study with 80 parents of children with leukemia; all of the caregivers were mothers and were included in the research. They were chosen using the convenience sampling method and categorized randomly into two groups (intervention and control, each with 40 participants). Research tools included demographic information and Quality of Life (QoL) scale (a specific form for first-degree family caregivers of patients with leukemia). Questionnaires were applied at the beginning of the research and after three months; this second evaluation coincided in the study group with the end of the intervention. The intervention lasted four sessions of 45- to 65-minute classes, with lectures and question and answer sessions, educational booklets, and posters in groups with 4 - 6 members. Results. In the first evaluation, mean QoL scores in the study and control groups were 224.9 and 225.7, which, after three months changed to 338.2 and 226.7, respectively. T-test verified these increases (p<0.05) in quality of life in the intervention group. Conclusion. According to the effectiveness of the education on the parent's quality of life, it is recommended to implement consulting and educational programs for parents, especially parents of children with leukemia, to promote care, reduce anxiety, and consequently, enhance quality of life.

Objetivo. Investigar el impacto de la educación en la calidad de vida de padres y madres de menores con leucemia referidos a la clínica de oncología del hospital Afzali-Poor en Kerman (Irán). Metodología. Estudio intervencional con 80 padres de menores con leucemia; todos los cuidadores de familia fueron madres y se incluyeron en la investigación. Fueron seleccionados utilizando el método de muestreo por conveniencia y asignados aleatoriamente en dos grupos (intervención y control, cada uno con 40 participantes). Las herramientas de investigación incluyeron información demográfica y la escala de calidad de vida (formulario específico para familiares de primer grado de consanguinidad cuidadores de menores con leucemia). Las encuestas se aplicaron al inicio de la investigación y luego de tres meses; esta segunda evaluación coincidió en el grupo de estudio con el final de la intervención. La intervención se hizo en cuatro sesiones de clases de 45 a 65 minutos, con conferencias y sesiones de preguntas y respuestas, cartillas educacionales y afiches en grupos con 4 - 6 integrantes. Resultados. En la primera evaluación, las puntuaciones promedio basales de calidad de vida en los grupos de estudio y control fueron 224.9 y 225.7, que, luego de tres meses cambiaron a 338.2 y 22.7, respectivamente. La prueba t verificó estos incrementos (p<0.05) en calidad de vida en el grupo de intervención. Conclusión. Según la efectividad de la educación sobre la calidad de vida de los padres, se recomienda implementar programas educacionales y de consultoría para los padres, específicamente los padres de menores con leucemia, para promover cuidados, reducir ansiedad y, en consecuencia, mejorar la calidad de vida.

Objetivo. Pesquisar o impacto da educação na qualidade de vida de pais e mães de menores com leucemia referidos à clínica de oncologia do hospital Afzali-Poor em Kerman (Irã). Metodologia. Estudo intervencional com 80 pais de menores com leucemia; todos os cuidadores de família foram mães e se incluíram na investigação. Foram selecionados utilizando o método de amostragem por conveniência e atribuídos aleatoriamente em dois grupos (intervenção e controle, cada um com 40 participantes). Ferramentas de investigação incluíram informação demográfica e a escala de qualidade de vida (formulário específico para familiares de primeiro grau de consanguinidade cuidadores de menores com leucemia). As enquetes se aplicaram ao início da investigação e depois de três meses; esta segunda avaliação coincidiu no grupo de estudo com o final da intervenção. A intervenção durou por quatro sessões de classes de 45 a 65 minutos, com conferências e sessões de perguntas e respostas, cartilhas educacionais e cartaz em grupos com 4 - 6 integrantes. Resultados. Na primeira avaliação, as pontuações média de qualidade de vida nos grupos de estudo e controle foram 224.9 e 225.7, que, depois de três meses mudaram a 338.2 e 226.7, respectivamente. A prova t verificou estes incrementos (p<0.05) em qualidade de vida no grupo de intervenção. Conclusão. Segundo a efetividade da educação sobre a qualidade de vida dos pais, recomenda-se implementar programas educacionais e de consultoria para os pais, especificamente os pais de menores com leucemia, para promover cuidados, reduzir ansiedade e, em consequência, melhorar a qualidade de vida.

Phytotoxicity and stimulatory impacts of nanosized and bulk titanium dioxide on fennel (Foeniculum vulgare Mill).

The objective of the this study was to compare concentrations of nanosized TiO2 at 0, 5, 20, 40, 60 and 80 mg L(-1) with bulk TiO2 for phytotoxic and stimulatory effects on fennel seed germination and early growth stage. After 14 d of seed incubation, germination percentage highly improved following exposure to 60 ppm nanosized TiO2. Similar positive effects occurred in terms of shoot dry weight and germination rate. Application of bulk TiO2 particles in 40 ppm concentration greatly decreased shoot biomass up to 50% compared to the control. Application of 40 ppm nanosized TiO2 treatment improved mean germination time by 31.8% in comparison to the untreated control. In addition, low and intermediate concentrations of nanosized TiO2 enhanced indices such as germination value, vigor index and mean daily germination. In general, there was a considerable response by fennel seed to nanosized TiO2 presenting the possibility of a new approach to overcome problems with seed germination in some plant species, particularly medicinal plants.

A new chromosomal phylogeny supports the repeated origin of vectorial capacity in malaria mosquitoes of the Anopheles gambiae complex.

Understanding phylogenetic relationships within species complexes of disease vectors is crucial for identifying genomic changes associated with the evolution of epidemiologically important traits. However, the high degree of genetic similarity among sibling species confounds the ability to determine phylogenetic relationships using molecular markers. The goal of this study was to infer the ancestral-descendant relationships among malaria vectors and nonvectors of the Anopheles gambiae species complex by analyzing breakpoints of fixed chromosomal inversions in ingroup and several outgroup species. We identified genes at breakpoints of fixed overlapping chromosomal inversions 2Ro and 2Rp of An. merus using fluorescence in situ hybridization, a whole-genome mate-paired sequencing, and clone sequencing. We also mapped breakpoints of a chromosomal inversion 2La (common to An. merus, An. gambiae, and An. arabiensis) in outgroup species using a bioinformatics approach. We demonstrated that the "standard" 2R+(p) arrangement and "inverted" 2Ro and 2La arrangements are present in outgroup species Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus. The data indicate that the ancestral species of the An. gambiae complex had the 2Ro, 2R+(p), and 2La chromosomal arrangements. The "inverted" 2Ro arrangement uniquely characterizes a malaria vector An. merus as the basal species in the complex. The rooted chromosomal phylogeny implies that An. merus acquired the 2Rp inversion and that its sister species An. gambiae acquired the 2R+(o) inversion from the ancestral species. The karyotype of nonvectors An. quadriannulatus A and B was derived from the karyotype of the major malaria vector An. gambiae. We conclude that the ability to effectively transmit human malaria had originated repeatedly in the complex. Our findings also suggest that saltwater tolerance originated first in An. merus and then independently in An. melas. The new chromosomal phylogeny will facilitate identifying the association of evolutionary genomic changes with epidemiologically important phenotypes.

An integrated chromosome map of microsatellite markers and inversion breakpoints for an Asian malaria mosquito, Anopheles stephensi.

Anopheles stephensi is one of the major vectors of malaria in the Middle East and Indo-Pakistan subcontinent. Understanding the population genetic structure of malaria mosquitoes is important for developing adequate and successful vector control strategies. Commonly used markers for inferring anopheline taxonomic and population status include microsatellites and chromosomal inversions. Knowledge about chromosomal locations of microsatellite markers with respect to polymorphic inversions could be useful for better understanding a genetic structure of natural populations. However, fragments with microsatellites used in population genetic studies are usually too short for successful labeling and hybridization with chromosomes. We designed new primers for amplification of microsatellite loci identified in the A. stephensi genome sequenced with next-generation technologies. Twelve microsatellites were mapped to polytene chromosomes from ovarian nurse cells of A. stephensi using fluorescent in situ hybridization. All microsatellites hybridized to unique locations on autosomes, and 7 of them localized to the largest arm 2R. Ten microsatellites were mapped inside the previously described polymorphic chromosomal inversions, including 4 loci located inside the widespread inversion 2Rb. We analyzed microsatellite-based population genetic data available for A. stephensi in light of our mapping results. This study demonstrates that the chromosomal position of microsatellites may affect estimates of population genetic parameters and highlights the importance of developing physical maps for nonmodel organisms.